In vitro reconstruction of the Aspergillus (5 Emericella) nidulans genome
نویسندگان
چکیده
A physical map of the 31-megabase Aspergillus nidulans genome is reported, in which 94% of 5,134 cosmids are assigned to 49 contiguous segments. The physical map is the result of a two-way ordering process, in which clones and probes were ordered simultaneously on a binary DNAyDNA hybridization matrix. Compression by elimination of redundant clones resulted in a minimal map, which is a chromosome walk. Repetitive DNA is nonrandomly dispersed in the A. nidulans genome, reminiscent of heterochromatic banding patterns of higher eukaryotes. We hypothesize gene clusters may arise by horizontal transfer and spread by transposition to explain the nonrandom pattern of repeats along chromo-
منابع مشابه
In vitro reconstruction of the Aspergillus (= Emericella) nidulans genome.
A physical map of the 31-megabase Aspergillus nidulans genome is reported, in which 94% of 5,134 cosmids are assigned to 49 contiguous segments. The physical map is the result of a two-way ordering process, in which clones and probes were ordered simultaneously on a binary DNA/DNA hybridization matrix. Compression by elimination of redundant clones resulted in a minimal map, which is a chromoso...
متن کاملFirst report of Emericella echinulata as a teleomorph of Aspergillus from Iran
During 2009–10, the presence of ascomycetous species in soil was studied in Fars province. Isolates were recovered from soil using modified Czapek (Raper et al. 1973), malt extract agar (MEA) and potato dextrose agar (PDA). Teleomorphic characteristics such as morphology of ascocarps, asci, ascospores and anamorphic characteristics such as conidiophores, phialides, conidia and chlamydospores we...
متن کاملCase Report. Maxillary sinus infection due to Emericella nidulans.
Fungal infections of the maxillary sinus are frequently caused by Aspergillus species, particularly A. fumigatus. In otherwise healthy persons there is an association with overfilling of dental root canals, when zinc-containing filling materials were used. Below, a maxillary sinus aspergilloma is reported in a young immunocompetent female patient caused by Aspergillus (Emericella) nidulans.
متن کاملModelling the Survival of Escherichia coli O157:H7 on Raw Portioned Tomatoes, Inoculated with Aspergillus fumigatus and Emericella nidulans
The metabiotic interactions occurring among two fungi (Aspergillus fumigatus and Emericella nidulans) and Escherichia coli O157:H7 on raw portioned tomatoes were studied. Tomatoes, preinoculated with the moulds and inoculated with the pathogen, were packaged in air and stored at 4, 8 and 12( composite function)C for 9 days; pathogen cell number and pH were monitored throughout the storage and t...
متن کاملEffect of Some Food Preservatives on the Lipolytic Activity of Beef Luncheon Fungi
Beef luncheon meat is one of the most popular meals in several countries in the world including Egypt. Thirty one fungal species and 3 species varieties were recovered from 30 samples of beef luncheon meat collected from different supermarkets in Qena. Alternaria, Aspergillus, Emericella, Mucor, Mycosphaerella, Penicillium and Rhizopus were the most common genera on the two types of media. From...
متن کامل